Temporal filtering retained frequencies below 0.08 Hz, spatial smoothing was performed using a 4-mm full-width half maximum Gaussian blur.
To avoid problems associated with very low absolute amounts, we retained frequencies that comprised at least 6 and 17 events in the case of first and overall choices respectively.
A low-pass temporal filter removed constant offsets and linear trends over each run while retaining frequencies below 0.08 Hz.
Table 2 Final retained classifiers Frequency Number of genes Cross validation Final retained genes At least 4 times 2 genes 91.7 gene456, gene626, gene672, gene874, gene907, gene918, gene951, gene956, gene979, gene1001 gene1001 At least 3 times 4 genes 96.7 At least 2 times 11 genes 98.3.
Table 6 Final retained classifiers Frequency Number of genes Cross validation Final retained genes At least 4 times 1 genes 92.1 gene2619, gene1495, gene2425, gene2746, gene4849, gene1788, gene1897, gene2848, gene4155 At least 3 times 5 genes 91 At least 2 times 9 genes 95.5.
Aside from these signals most peaks of ghrelin retained their frequencies in the presence of the membrane-mimetic, implying that most residues of ghrelin remain in the aqueous phase.
Alleles that flow freely will have their frequencies homogenised across species, while alleles whose introgression is blocked by divergent selection will retain strong frequency differences.
This synthetic set of reads retains the frequency and relative distribution of nucleotides from the observed data.
Unlike assemblers optimized for large repetitive genomes, VICUNA aggressively merges similar sequences, and has the capacity to retain low frequency single nucleotide and length polymorphisms.
A shuffling test (See Method for details) shows that no more than 400 TA-led genes would be identified in random sequences retaining dinucleotide frequency.
