The first level trigger logic successfully kept the trigger rate within the permissible bounds when challenged with artificially produced as well as naturally encountered night sky background fluctuations and while retaining events with general air-shower characteristics.
The bias to retain events with correlated expression patterns may favor the retroposition of genes that have embedded regulatory elements or leaky transcription from upstream regions, although we believe the latter to be uncommon.
Similarly, up to 18 R2R3-MYB genes in Arabidopsis undergo similar AS events, including five multiple promoters events, five competing 5′ splice sites events, four competing 3′ splice sites events, one multiple poly(A) sites event, and one retained intron event.
In our analysis, we found that the last exon - ENSMUSE00000643467 (Cexhibitedibited retained intronic event with high PSI values in post-natal developmental stages compared to embryonic stages, with a very low abundance in E15 as shown in Fig. 5c.
Finally, the results of the most prominent splicing events namely skipped exon and retained intron events are also made available through Eye splicer, a web based splicing browser showing developmentally altered splicing events in mouse lens.
Functional analysis of the genes exhibiting the most abundant alternative splicing events, namely skipped exon and retained intron events, revealed the enrichment of mRNA processing, apoptotic signaling pathways, protein polymerization, cell development and differentiation for the former and the enrichment of cell cycle processes, stress and splicing regulation for the later type of events.
The most prominent splicing event types namely skipped exon and retained intron events were made available through Eye splicer (http://www.iupui.edu/~sysbio/eye-splicer/), a web based splicing browser showing developmentally altered splicing events in mouse lens.
Our splicing analysis indicated that retained intron events are the second most abundant alternative splicing events after skipped exon events (Table 3).
Since skipped exon and retained intron events were the most abundant, we collected these events from raw rMATS outputs specifically those which are supported by reads that span splicing junctions and reads on target below 1% FDR.
Functional analysis of the genes associated with high confident retained intron events across lens developmental stages.
