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The sensor shows a low Michaelis-Menten constant value of 2.4 μM indicates the high affinity of enzyme to the analyte points to the retained activity of enzyme after immobilization.
Similar to native unfused ClyA, chimeric ClyA fusion proteins were found localized in bacterial OMVs and retained activity of the fusion partners, demonstrating for the first time that ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs.
The excellent electrochemical performance of the HRP-TNS electrode was attributed to biocompatibility of the titanate sheets, porous architectures of the HRP-TNS film which retained activity of HRP to large extent, avoided aggregation of HRP, provided better mass transport and allowed more HRP loading per unit area.
Intermediate calculated values needed for the determination of the retained activity of CLEAs have been summarized.
Figure 1 schematizes the protocol we hereby propose for the calculation of the retained activity of CLEAs based on the comparison of specific activities of CLEAs and the specific activity of free enzyme.
Nevertheless, there is now convincing evidence that AMPs, such as similarly salt-sensitive defensins and chemokines[57], [58] contribute to enhanced bacterial killing in vivo, likely reflecting the necessity of their compartmentalization, presence of ionic microenvironments (as illustrated by retained activity of PrP in sweat buffers), or synergism between AMPs.
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These results were surprising, because the presence of an m-trifluoromethyl group was previously shown to be important to retain activity of related kinase inhibitors but, as shown here, is not essential for activation of IRE1α-KR43.
These studies have clearly demonstrated role of formulation in retaining activity of biologics.
The homing, recruiting, organising and retaining activities of chemokines is dependent on the presence of and interaction with chemokine receptors on the leukocyte surface.
We show that VEGF-CPG2 fusion proteins retain activities of both parental molecules, irrespective of whether they are the N- or C-terminal moiety, and that they retain these characteristics if either very short or longer linkers are used to join them.
Both carriers retained antitumor activity of DAS and could form mixed micelles with DOX.
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