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The retained activities in ι-carrageenan beads were measured for both free FSAwt and immobilised FSAwt revealing a 3 fold higher activity in favour of the pre-immobilised FSA.
The count rates were normalized to the value immediately post-administration (taken as 100%) to yield relative retained activities (in %).
We retained activities that showed significant skew towards high altruism ratings and a modal rating of 4 or 5; the results of this analysis and the activities used in the main experiment are given in Additional file 1: Table S1.
When Tyr-142 was replaced with phenylalanine, the resultant Y142F mutant retained activities towards both aromatic and aliphatic nitrile substrates, with substrate specificity similar to that of the wild-type enzyme.
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Highly active compounds also retained activity against H. capsulatum phagocytosed into P388D1 macrophages.
Thermal and storage stability for immobilized invertase were found to be 35 °C for 60 min (85% retained activity) and 120 days storage period (80% retained activity), respectively.
Moreover, lyophilized reaction mixtures retained activity for prolonged periods under dry conditions at room temperature.
BLBLIs or carbapenems also retained activity against Gram-positive anaerobes (99.5%100%%) except Clostridioides difficile.
Thus, several strategies are employed to intentionally display ligands at interfaces with retained activity.
The biocatalysts retained activity better when stored in milk at 4°C than in 50% glycerol.
Domain D favoured lipophilic residues and domain E retained activity when the carboxylic acid was esterified.
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