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However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage.
Mad was strongly expressed in differentiating suprabasal keratinocytes in healing dermal wounds and in benign hyperproliferative conditions, but also in squamous cell carcinomas, in which the keratinocytes retain their differentiation potential.
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The upscaled WJ-MSCs retained their differentiation potential and immunosuppressive capacity.
Placing the inner cell mass in vitro under the finely tuned culture conditions prevents their imminent differentiation and allows cells to self-renew seemingly indefinitely whilst retaining their differentiation potential (Thomson et al., 1998).
To overcome this problem, we attempted to prolong the life span of human placenta-derived mesenchymal cells (hPDMCs) by modifying hTERT and Bmi-1, and investigated whether these modified hPDMCs retained their differentiation capability and multipotency.
Alternative differentiation is observed in human keratinocytes lacking p16INK-4a, expressing telomerase and retaining their differentiation capacities (N-hTERT cells) upon sublethal repeated exposures to UVB [7].
Repeated exposures to sublethal doses of UVB induce an alternative differentiation state rather than premature senescence in cultivated human keratinocytes lacking functional p16INK-4a, immortalized with telomerase and retaining their differentiation capacities (called N-hTERT cells herein) [7], [8].
We demonstrated that young MSCs retained their differentiation potential (measuring adipogenic differentiation; data not shown), but that HBO had no effect on this process.
At present it is unclear if the cultured spermatogonial cells retain their full differentiation capacity of spermatogenesis after transplantation.
If this effect was due to stem cell self-renewal, we would predict that cells derived from these spheres would retain their multipotent differentiation ability.
MSCs carrying two kinds of nanoparticles [ 23] (polylactic acid NPs and LNCs) were shown to retain their viability, differentiation, and tumor homing capacities [ 22, 24].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com