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Although we see that the cell lines retain the expression of most genes, the differentially expressed genes between tumors and cell lines are very important to investigate while we translate our discovery from in vitro to in vivo.
In summary, results from various laboratories worldwide indicate that hCMEC/D3 cells retain the expression of most transporters and receptors expressed in vivo at the human BBB, including MDR1, BCRP, MRP4, transferrin receptor, insulin receptor, Glut-1; they also express metabolizing enzymes and TJ proteins, as expected.
Then, let us retain the expression of (alpha_{1}) and introduce the following error: e_{2} = hat{z}_{3} - alpha_{1},.
Thus CD146hi stromal cells from primary cultures retain the expression profile associated with the HSC niche.
Because TGCTs and fetal germ cells retain the expression of the "ESC-specific" alkaline phosphatase enzyme visualized by NBT BCIP and Kiel, these staining protocols can be used to stain TGCTs [18], fetal gonocytes and oogonia [15], [16], and other tumours and ESC-like cells with ESC-like properties expressing alkaline phosphatase [14], [18].
In addition, PDAC cannibal cells retain the expression of epithelial markers, suggesting that these cells did not achieve EMT.
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Neural precursors were mechanically isolated and cultured in suspension as neurospheres, which extinguished the expression of pluripotent factors NANOG, LIN28 and OCT4 while retaining the expression of SOX2, a transcription factor expressed in neuronal precursors (Fig. 2B).
FF-hESCs retained the expression of the pluripotent marker Oct-4, a POU domain transcription factor exclusively expressed in undifferentiated hESCs (Figure 1A).
Co-cultures on D-PHI released more IL-10 (anti-inflammatory) than monocytes cultured alone, while the VSMCs retained the expression of its marker protein calponin.
Monocytes stained with the lectins wheat germ agglutinin (WGA) and peanut agglutinin (PNA), but not soybean agglutinin (SBA), whereas macrophages and fibrocytes retained the expression of WGA, but did not stain with PNA or SBA (data not shown).
Flow cytometric analysis disclosed that 20 40% of expanded human VPC-derived EC retained the expression of the endothelial cell-related markers, including VE-cadherin, VEGFR2, CD34, CD31 and CD105, and all of the cells were negative for a panleukocyte marker CD45, monocyte/macrophage marker (CD11b), and stem/progenitor makers (AC133 and c-kit) (Figure 1a).
More suggestions(14)
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conserved the expression
maintaining the expression
conserving the expression
conservation the expression
maintains the expression
maintain the expression
maintained the expression
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com