Like I-DmoI, H-DmoC2 is thermostable and cleaves the new target DNA to generate the predicted 4 nt 3′-OH overhangs but, unlike I-DmoI, H-DmoC2 retains stringent cleavage specificity when substituting Mn2+ for Mg2+ as co-factor.
The FDA proposal would establish a minimum standard that would apply nationwide, but states could retain more stringent requirements if they wished.
Given the nature of the study, these data are likely to reflect real world blood pressure management, and any consideration of retaining more stringent blood pressure targets (particularly if low values are proved to be unnecessary in those with pre-existing cardiovascular disease 8) has to consider the impact of at least aiming for lower blood pressure to achieve values ≤140/90 mm Hg.
2. It should also be possible to authorise parenteral administration, eye drops and nasal sprays under the simplified registration procedure, whilst retaining the stringent test criteria for quality and safety.
Regardless, the Adam Walsh Act sets only the minimum guidelines; many states will retain their own, more stringent community-notification laws for juveniles.
Because, for teamed-up philopatric males specialized in the defense and expansion of a common territory so as to attract and retain exogamic females, the stringent requirements of team solidarity in exterior politics (between-team antagonistic competition) select for strictly inhibited, bloodless and weaponless combats in interior politics (within-team antagonistic competition).
A total of 653 probesets were retained at a stringent cutoff of p<0.0001 and fold-change of 4. Probesets were centered by mean expression values, and hierarchical clustering was performed by complete linkage and uncentered correlation as the similarity metric using Cluster 3.0 program.
Because many of the genomes are not assembled yet, we used stringent criteria to retain a sequence for analysis.
These motifs were initially discarded from the automatic procedure due to the stringent parameterization to retain only the highest-scoring motifs for the specified position.
We used stringent criteria to retain a sequence for analysis and the analyses restricted to the five annotated strains, each of which have at least 85% of their bases with a quality score greater or equal to 40, to confirm the robustness of our conclusions.
However, for the human genome, the minimal amount of data that is necessary under stringent conditions to retain a full TinT resolution is about 10% of the genome, for instance about 300,000 traces are sufficient to receive the representative full TinT pattern.
