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You can choose whatever you want, but should probably stick to the deafault color palette above and consistently retain labels.
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The intensity of fluorescence of nuclear DNA of neurons, 1s/2s cells and epithelial cells that retained label and those that did not retain label was calculated after subtracting background intensity.
Those cells that retain label for long periods are likely those stem/progenitor cells that become quiescent behind the growth front.
By contrast, stem cells, which divide infrequently, retain label for long periods of time, much longer than the surrounding non-stem cells.
Using cell labeling techniques to monitor cell division history, it has been observed that hierarchically upstream stem cells with long-term self-renewal potential divide less frequently (i.e. retain label) than their downstream progeny (i.e. which dilute label) (Blanpain et al., 2004; Wilson et al., 2008; Foudi et al., 2009).
We did this by injecting either l-[methyl-3H]-methionine or l-[35S]-methionine into dwarf or normal mice and then determined retained label (in form of S-adenosylmethionine) 45 min later.
Hydra were simultaneously macerated to identify the cell types that retained label, as described (David, 1973).
Ectodermal and endodermal epithelial cells retained label longer, for four weeks and sometimes until five weeks.
Passing unlabeled liposomes resulted in an elution of both retained labeled lipids and calcein.
Many of these gland cells possibly retained label due to terminal differentiation.
The cells retaining label were defined as those which had EdU signal that completely colocalized with signal from DAPI staining of nuclear DNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com