It was evident from the result that DNA after releasing from DNA-PLA-PEG-NPs still retained gene expression activity.
We refer to this as the LARGE (Lost, Acquired, Retained Gene Expression) Principle of Cellular Reprogramming.
The retained gene expression sets included genes like PKNOX2 (AFiPSCs); HMBOX1, MGA (FiPSCs) or RAXL1 (AFiPSCs & FiPSCs).
We refer to this as the LARGE (Lost, Acquired and Retained Gene Expression) Principle of Cellular Reprogramming.
We discuss a fundamental principle of cellular reprogramming, which we have coined LARGE, the Lost, Acquired and Retained Gene Expression principle.
At 0.5 h after transfer to either Medium M or T, the cells initially retained gene expression patterns, staying around Attractor W. Thereafter, in Medium M the distribution of cells shifted toward Attractor 1.
By defining distinct and overlapping gene expression patterns and deriving the LARGE (Lost, Acquired and Retained Gene Expression) Principle of Cellular Reprogramming, we could further highlight that AFiPSCs, FiPSCs and ESCs share a core self-renewal gene regulatory network driven by OCT4, SOX2 and NANOG.
Different duplicate retained gene expression levels were recently reported to be partially a result of epigenetic modifications such as methylation, histone modification, small RNAs, and transposable element genes (review in [ 43]).
Taken together, our analyses demonstrate that BC cell lines retain methylation dependent gene expression patterns observed in basal-like BCs, and strengthen an epigenetic influence on some BC phenotypes that are retained in their equivalent model systems.
Above all, it is not clear if cells in culture retain the gene expression profiles of their in vivo counterparts.
