However, some variants retained functioning similar to the wild type.
This phenomena relates to the redundancy of biological systems which are to ensure that the system retains functioning inspite of the perturbations.
"The premise of orthopedics is to restore alignment so you can retain function," says Lieberman.
The I464A and R466A mutants retain function, though mutation of D469 to alanine inactivates the protein, both in terms of checkpoint function and catalytic activity.
Subfunctionalisation can also occur if parental and duplicates retain function but become distinct and complementary in their spatiotemporal pattern of expression.
First, in armXM19 (M/Z) mutants, the truncated ArmXM19 protein does not activate the Wg signal in the nucleus, but does retain function in the adherens junction[31].
The remaining constrained sites retain function and evolve at the same dependent rate in all groups.
We argued that for the truncated forms to retain function, they should localize similarly to the full-length proteins.
Consistent with this idea, OxlT mutants retain function when the R1 labels are placed at locations more distant from the permeation pathway.
The resulting truncated protein would lack the 5 C-terminal transmembrane helices of the MFS domain and thus be very unlikely to retain function.
