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The chain heating parameter was set at 0.3 and apart from the model settings, the default settings were retained.
The default settings were retained for all parameters, with the exception of the matrix (BLOSUM series) used for both pairwise and multiple alignments.
Contigs were assembled with Lasergene SeqMan II (DNA-Star) 4.03 and aligned using MUSCLE 3.6 [44] (default settings were retained except maximum number of iterations (maxiters) = 1000).
Variants were filtered with vcfutils "varFilter" using default settings except retaining all variants with under 99999 reads.
The 13 amino acid data were concatenated as an alignment of 3819 positions after individually aligned, and then, 2520 aligned characters for 24 taxa and 2616 aligned characters for 21 taxa were retained respectively after Gblocks screening with default settings [ 70].
DAPC was implemented in R v3.0.1 [ 83] using adegenet v1.3.1 [ 84]. adegenet's find.clusters function was used to estimate the ideal value of K (default parameters, retaining all principal components), and optim.a.score was used to estimate the ideal number of principal components to retain in the final discriminant analysis (using default settings and 25 full simulations).
Sequences were assembled and edited using Sequencher 4.9 (Gene Codes) and aligned using MEGA 4 (Tamura et al. 2007) using default settings, and checked manually to assure the reading frame was retained.
Default settings were used.
with default settings.
New research on default settings.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com