Indeed we observe that although most cell cycle protein complexes retain cell cycle-regulated genes in both species, the identity of dynamic members differs between species (Additional file 3: Figure S4).
The ability of Bcl-2 to retain its cell cycle inhibitory affect on splenic T cells in the absence of p27 was surprising.
This demonstrates that a large proportion of the [H]-thymidine retaining cell was traversing the cell cycle when 5BrdU was given.
cds1Δ cells retain the cell-cycle arrest function, because, in the absence of Cds1, Rad3 can arrest cells via Chk1.
Rat Schwann cells expressing SV40LT proliferate in the absence of mitogens but retain other cell-cycle checkpoints such as anchorage dependence and contact inhibition of proliferation.
Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics.
As mentioned above, [H]-thymidine retention defines the long label retaining cells in group 4, and 5BrdU incorporation defines the cells traversing the cell cycle when this label was administered on days 11 and 12 of pregnancy.
In two further clades, both Vpr and Vpx are present with Vpr retaining only cell-cycle arrest activity and Vpx only inducing SAMHD1 degradation.
Therefore the use of more relaxed criteria could well have retained more new putative cell cycle genes.
Group 4 was intended to define long label retaining ([H]-thymidine-positive) alveolar epithelial cells that traverse the cell cycle while retaining their original [H]-thymidine label.
