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Complex ethical issues arise due to the vulnerability of patients and families, difficulties interpreting negative results, restriction of communication to "yes" or "no" answers, and cost.
These ethical issues arise due to difficulties interpreting negative neuroimaging results, restriction of communication to "yes" or "no" answers, the vulnerability of patients and families, and the cost of fMRI.
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The process of Query Tunneling is divided into two main steps, Query Relaxation to make a higher order query suitable to a restricted interface and Result Restriction in order to filter the results using the original query.
The resulting restriction digestion pattern shown in Figure 7A demonstrates that this cDNA is indeed maternally expressed.
The resulting restriction fragments were ligated to adaptor set TA-1b as previously described [22], except that incubations were carried out at 16°C for 16 hr.
The resulting restriction fragments were separated by PFGE using the MAPPER system (Bio-Rad Laboratory, HerCAles, CA, USA).
The resulting restriction fragments were separated by electrophoresis on an agarose gel (4percentt) using 25- and 50-bp molecular-weight markers (Invitrogen™).
Although the resulting restriction fragments will differ, we expect a priori that the overall genome architecture should be the same from such replicate experiments.
The resulting restriction fragments were separated electrophoretically on 8% polyacrylamide gels and visualized under UV light after ethidium bromide (Et-Br) staining.
The resulting restriction profiles showed high similarities among digestate, MFC-C, and MFC-U samples and between the MFCs and G. sulfurreducens.
The resulting restriction profile was nearly identical across the plasmids except for pYHCC from YD472, which had a shift of a band from ≈8 kb to ≈11 kb.
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