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Utilizing coexpression with the bacterial selA, selB, and selC genes (encoding selenocysteine synthase, SelB, and tRNASec, respectively) the yield of recombinant seleno-GST was about 2.9 mg/L bacterial culture, concomitant with formation of approximately 85% truncation product as a result of termination of translation at the selenocysteine-encoding UGA codon.
Mutation of histone RNA elements or disruption of the processing machinery results in the polyadenylation of replication-dependent histone mRNAs, indicating that these mRNAs acquired polyadenylation signals, likely as a result of termination read-through.
If the expression of VP2fluc is a result of termination-reinitiation, translating ribosomes would be unable to reach the AUG start codon of VP2fluc in the mutant mRNA and the ORF could not be translated.
Other countries using the test have had an increase in the rate of post-positive-result terminations.
Finally, caveolae-mediated uptake of receptors results in termination of signaling or facilitates intracellular signaling [ 3].
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This result prompted early termination of the trial.
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CEO of Professional Science Editing for Scientists @ prosciediting.com