The resulting insert was excised by digestion with BamHI and KpnI, and ligated along with a kanr cassette containing KpnI XhoI ends into the BamHI XhoI sites of the pPROEX-HTb vector (Invitrogen).
In the secondary reaction, the resulting insert of choice would anneal with the homologous sequence in the target plasmid for extension to generate the long fused fragment.
RESULT Inserted the phrase into a droll bit about an anatomically themed truck accessory she had seen down South that was intended to make pickups look more masculine.
The result inserted in Fig. 7a is the EDS spectra taken from Fig. 7a.
As a result, inserts with P = 0.4 mm achieve the best PEC values (performance evaluation criterion).
The orientations of the resulting inserts were determined by restriction digests with Sac I.
Many of the resulting inserts were small fragments that include little or no recognisable mobile element sequence.
As a result, insert-related caller-specific uncertainties in the locations of SV breakpoints are likely averaged out in our analysis procedure.
In these cases, the sequence assemblies frequently contain too many contigs that cannot be unequivocally ordered even with fosmid-derived read-pairs (in part because the fosmid insert is too large, with the resulting insert-end reads simply skipping over many contigs and gaps).
