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Western blot results for expression of vascular endothelial cadherin and occludin also showed decreased expression of junctional proteins.
Moreover, we found similar results for expression variability and for noise.
The results for expression breadth confirmed its positive relation with both UTRs and introns whereas the correlations for CDS again differed between MPSS (positively correlated) and oligo-array (negatively correlated).
For three other genes, we obtained disparate results for expression when the control and MS samples were pooled for analysis.
The same difficulties are encountered when interpreting the results for expression levels of cyclin D1 and TGF-β3.
The results for expression of genes that were not significantly regulated after syngeneic transplantation according to the array were set to 1.
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Additional file 3: Wilcoxon Signed Rank test result for expression value and tissue specificity between relic and its orthologous counterpart.
We note that all methods are superior to the BHC result for expression data alone (BHI = 0.323), except for the ChIP only analyses.
In line with the IHC results for protein expression, EGFR mRNA expression levels showed no significant correlation to overall or progression free survival.
For example, Kreth and colleagues [ 12] found that patients with discordant results for MGMT expression (determined by mRNA expression) and methylation status did worse than concordant counterparts.
Results for the expression of each cytokine were normalized to expression and both cytokine and β-actin were measured in each sample.
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