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As shown, DIEA afforded the best results (entry 7).
The combination of DMF for solvent A, and MeCN for solvents B and C afforded the best results (entry 7).
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Interestingly, the use of H2O as either solvent A or C afforded comparable results (entries 5, 8, and 10).
That is, if the acceptance rate for men was 100%, the resulting entry rate for men would still be below that of women," the report said.
The final result for each element is appended along with the calculated probabilities for each class in a result entry.
Resulting entry vectors were LR recombined with pYES-DEST52.
The resulting entry clone was jointly recombined with pENTR-L1-Kozak-Venus-Stop-L2 into pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5
After the DNA sequence of the resulting entry plasmid has been verified, tetR-10, Pmyc1tetO, and the target gene are cloned into a destination plasmid by LR recombination (Figure 1.A).
Expression constructs, generated by LR recombination of the resulting entry clones into pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 pSP72-R3-ccdB-CmR-R5 B1-Kozak-NLS-LacZ-Stop-B2 B1-Kozak-NLS-LacZ-Stop-B2 B1-Kozak-NLS-LacZ-Stop-B2 B1-Kozak-NLS-LacZ-Stop-B2 B1-Kozak-NLS-LacZ-Stop-B2 B1-Kozak-NLS-LacZ-Stop-B2
In both cases, significant biphenyl formation was observed, which was a typical result (entry 16).
The resulting entry pDONR207- clones were selected on gentamycin plates and validated by restriction enzyme digestion.
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