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The resulting targeted mice were mated with transgenic CMV-Cre mice [ 15] to drive germline LoxP recombination, resulting in the null mutant alleles that were used for this study.
The resulting targeted MPIO show a highly effective binding with PMS-stimulated bEnd.3 cells, which resulted in significantly lower signal intensity in the T2-weighted images compared with the control nonstimulated cells.
The resulting targeted cells can be analyzed through the use of universal primers flanking the fragments and subsequent sequencing.
Linearized constructs were electroporated into RI ES cells, and resulting targeted ES cells used for C57/Bl6 blastocyst injections according to standard protocols.
The resulting targeted control and WGS alignments were used for variant calling using GATK v.3.3-0 withethe --stand_call_conf optoon set to 50, the --stand_emit_conf optoon set to 20, and the -dcov option set to 200.
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The resulting targeting construct replaced the exon 2 of the Rad21 gene, resulting in a null allele (Fig. S1A).
The resulting targeting constructs were confirmed by sequencing.
Yet the resulting target, of 2.6%, is artificial, since Poland and Sweden are not in the euro.
The resulting target identity is important from the viewpoint of practice and application.
The precursor copolymers were post-functionalized utilizing di-n-propylamine and the resulting target copolymers were fully characterized.
We describe a method to model the hot electron transport and deposition and the resulting target radiation-hydrodynamics.
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