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Enhanced adhesion was observed only at synthetic peptide concentrations above 500 µg/ml; however, when a 2% SDS extract was prepared from non-adhering bacterial cells plus the synthetic peptide, the resulting preparation conferred increased adhesion at lower synthetic peptide concentrations (Figure 10B).
The resulting preparation is often used as a flour substitute.
The resulting preparation was re-suspended in 1 ml of distilled water and stored until quantification.
The resulting preparation was creamy with each milliliter equaling 4.5 g herbs.
The resulting preparation was centrifuged (10 000 rpm, 10 min ., washed three times with ethanol, dissolved in distilled water, and lyophilized.
The resulting preparation contained much smaller amount of Dd (not shown), with HAWW_5 that localized in the heavy sucrose fractions containing Dd.
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The purity of the resulting preparations was evaluated by SDS-PAGE on 4 15% Tris-HCl gel (Bio-Rad).
The resulting preparations were scanned and photographed using an H-12 electron microscope (Hitachi, Japan).
The resulting preparations were at least 90% pure as judged by protein staining (GelCode Blue Stain Reagent (Pierce)) following sodium dodecylsulfate SDS -polyacrylamide gel electrophoresis.
FD is the coordinator of the project and helps for the interpretation of results, preparation and corrections of this paper.
GZ took an active part in the discussion of the results, preparation of the manuscript, and he coordinated the research.
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