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The heat sensitive psychrophilic protease subtilisin S41 was previously subjected to three rounds of mutagenesis/recombination and screening, resulting in variant 3-2G7, whose half-life at 60°C is approx. 500 times that of wild-type.
Structural variation was also assessed for SHS samples with paired end sequencing, resulting in variant identification.
Animals respond individually to various stress conditions, resulting in variant endocrine changes that could alter their ability to conceive.
We propose that the NADC30 strain was introduced into China in recent years by importing of breeding pigs and has since undergone mutations, resulting in variant viruses.
The two sub-genomes of Miscanthus are quite similar, resulting in variant frequencies among paralogs that are only modestly higher than those observed between alleles.
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Using selection from synthetic combinatorial libraries, we enhanced its affinity by > 100-fold, resulting in variants with Kd as low as 180 pM with no detectable loss of binding specificity.
Because Q823-P/S824-P displayed no loss of superactivity, the residue immediately N-terminal to the double-proline substitution was also permutated, resulting in variants containing three adjacent substitutions in the BH-HC hinge region.
Further, single-amino acid substitutions at Lys180 (i.e. RcsBK180A, RcsBK180R, RcsBK180Q) resulted in variant proteins that had two features.
Splice signals within HERVs can also result in variant transcripts of cellular genes.
Ecological barriers might result in variant divergence time between two insular groups (Taiwan and Hainan) from China mainland.
In Arabidopsis, clonal regenerants showed a range of mutations including base substitutions, insertions, and deletions that resulted in variant phenotypes [ 29].
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