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Visual inspection was conducted on the images corresponding to these genes to eliminate false positives resulting from technical issues such as variation in focusing and/or exposure time.
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Several reasons may result in the inability to identify a mutation using exome sequencing: False negatives can result from technical issues such as poor capture and subsequent low sequence coverage of exons, or from analytical errors such as from indiscriminant candidate filtering using SNPs from public databases.
However, as detailed in the supplement, this difference probably resulted from technical and/or analytical issues.
The audience quickly understood her lacklustre Grammys performance resulted from technical difficulties.
They emphasized that the delays resulted from technical problems, not lack of effort.
First of all, we cannot rule out that this issue resulted from a technical problem with the patch or glue.
In each case, standard deviations resulted from two technical replicates.
The low concordance between different studies results from the differences in technical issues, CNV calling algorithms as well as study populations [ 45], and can also indicate that numerous CNVs in the chicken genome are still expected to be discovered.
Apart from these technical issues, there are other confounding factors that may lead to false positive methylation results.
Aside from the technical issues, underlying this argument are valid concerns about corruption.
These results suggest that the technical issues with organellar and bacterial mRNAs that are not removed using rRNA depletion will not provide identical results as through conventional polyA+ selection, in which contamination from bacterial and organellar mRNAs is minimized.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com