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And after being fertilized and implanted into surrogate mothers, the resulting eggs produced apparently healthy mice like the one above.
He finally conceded, mating with two females towards the end of his life, but tragically, all of the resulting eggs failed to hatch.
The resulting eggs were collected and incubated until hatch under the conditions as described above.
The resulting eggs were left to hatch in liquid culture: 350 500 μl M9 buffer in a 24-well tissue culture plate.
The resulting eggs were allowed to hatch and develop for 3 days, during which time the progeny were moved to new plates for survival experiments.
Eclosed virgin adults with relatively severe outward modifications from each group were selected and kept in a cage for mating, and the resulting eggs were collected.
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Farahany explained that the donor of the additional mitochondria is not really a parent, considering that "99.9% of [the resulting egg's] coding DNA from the intending mother".
Similarly, you can add black pudding or chorizo to a scotch egg, use haggis, venison or chicken instead of pork, gild it with onion or apple, but while the resulting egg may rank somewhere on a sliding scale from "abomination" to "interesting alternative", none of those eggs will constitute a marked improvement on the best examples of the original pork version.
The resulting egg would then be implanted into the first woman using traditional IVF techniques.
We recovered large baz mutant clones expressing BazS980A-GFP only rarely, and the resulting egg chambers often had large gaps in the follicle cell layer, suggesting that mutant cells fail to integrate into the epithelium.
Under conditions of environmental stress such as seasonal changes, some females form eggs that undergo reductional division, resulting in eggs with the haploid number of chromosomes; such eggs must be fertilized by a male gamete to produce a new female.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com