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Our resulting assembly had 71 supercontigs of mean length 85,604 nucleotides, an N50 number of eight, and N50 length of 317,167 nucleotides; that is, the eight longest supercontigs were all at least 317,167 nucleotides long and together covered more than 50% of the predicted genome size of six megabases.
We report N50 and maximum contig length for each resulting assembly.
The resulting assembly contained 1,782 contigs and covered an estimated physical length of 0.93 Gb.
At higher number ratios the data analysis is unreliable because the resulting assemblies are in the form of few chains extending over the whole length of the microscope field (forming an effectively percolated structure).
After running several assemblies, we found that a K-mer size of 65 was giving the best results in terms of N50 contig and total assembly length after scaffolding and gap filling.
Hyb-Seq of 12 individuals (10 Asclepias species and two related genera) resulted in at least partial assembly of 92.6% of exons and 99.7% of genes and an average assembly length >2 Mbp.
We used a custom assembly workflow to optimize consensus genome map assembly, resulting in an assembly equal to the estimated length of the Tribolium castaneum genome and with an N50 of more than 1 Mb.
The resulting final assembly had a total length of 18.4 Mb and was composed of just 44 scaffolds.
The resulting draft assembly included 3,472 contigs with an average length of ~17 kb.
Co-assembly with Illumina paired-end sequences using Trinity substantially improved the assembly metrics, resulting in the assembly of more full-length transcripts.
The resulting genome assembly comprised of 32 scaffold sequences with a total length of 11,465,281 bp.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com