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Moreover, the cytoplasmic delivery of mRNA circumvented the nuclear envelope, which resulted in a higher gene expression level.
However, none of the three LNA modified ODNs resulted in a higher gene targeting efficiency than the PO or PS oligonucleotide.
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The plasmid paphG includes not just one copy of this hybrid aphVIII gene construct but 16 repeated aphVIII cassettes, this resulting in a higher gene dosage [ 22].
To reduce NHEJ, we note that inhibition of the DNA double-strand break (DSB) repair system was found to hinder the random gene integration process, resulting in a higher gene targeting efficiency by homologous DNA [ 27].
Three students in our sample explained the difference between low- and high-intensity pigmented plants, saying that low-intensity pigmentation resulted from plants inheriting fewer genes involved in anthocyanin biosynthesis, whereas high-intensity pigmented plants inherited more genes, resulting in a higher "gene dose".
Increasing the threshold tag number resulted in a higher number of genes detected by the globin reduced samples while the number of genes only detected in non-reduced samples remained approximately the same.
In addition, these data are listed in the Additional files 3 and 4. Interestingly, response to anti-CD3 alone resulted in a higher number of genes negatively regulated by PARP-1 than those regulated positively.
We then examined the transcriptome changes upon Ascl1ERT2 activation, which resulted in a higher number of regulated genes, possibly due to Ascl1 acting as a master and pioneering transcription factor (Wapinski et al., 2013).
Utilizing all probes per gene resulted in a higher threshold for determination of rhythmicity for a single gene within our database.
These values clearly indicate that exposure to a high BDE47 concentration resulted in a higher number of up-regulated genes.
To get an indication about whether environmental selection pressure resulted in a higher variability for loci close to genes related to health and fertility traits, we performed a gene enrichment analysis in an area of one Mb in either direction of segregating markers that were unique for each Korean breed (93 markers for Chikso, 212 markers for Jeju Black, and 1104 markers for Hanwoo).
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