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The cDNA sequence of 11 PMM genes (including seven PMM-1 and four PMM-2 members from bread wheat and its progenitor species) were aligned (Additional file 10), and the resultant alignment was used to construct a NJ tree (Additional file 10).
Based on the resultant alignment that was constructed using default parameters, the side chains were optimized and residues were minimized.
The resultant alignment of human ABCB1 and mouse ABCB1 sequences produced 87% sequence identity and 93% similarity.
The resultant alignment was then checked using JalView ver. 8.0 [62], totaling 595 bp in the final alignment (File S2).
The resultant alignment was edited by eye.
The resultant alignment of RNA-Seq reads was used to construct transcript units and predict SJs.
Similar(46)
The common 100-bp sequences extracted from both Daubentonia madagascariensis Aye-Aye 1 (DMand anDaubentoniaia madagascariensis Aye-Aye 2 (DMA2) satellite sequences were aligned using the MUSCLE program, and phylogenetic trees based on the resultant alignments were constructed using the Neighbor-Joining algorithm of PHYLIP software with F84 distance parameter (Edgar 2004; Felsenstein 2005).
The resultant alignments were revised by eye using amino acid alignments as the guide.
The resultant alignments were scored using the ALE program (Clark et al. 2013).
To determine the quality of the resultant alignments, we estimate their sensitivity and coverage.
The specificity and coverage of the resultant alignments are tested against the orthologous groups defined in the eggNOG database v3.0 [ 42].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com