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In addition, I'm not convinced that what the authors consider to be changes from microtubule minus-end to plus end localization or de novo plus end localization isn't simply a result of transcripts becoming anchored to the posterior cortex, such that they remain there despite changes in microtubule organization.
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However, 97 of these contigs do align to the reference genome of the WO1 strain, suggesting that these contigs are not the result of transcript misassembly or contamination of a foreign species, but instead that the SC5314 genome assembly is incomplete, and/or contains misassemblies.
Blastx results of transcripts to NCBI nr database, tabular format.
BlastX results of transcripts with the top 100 RPKM were extracted from the whole transcriptome blast against the NCBI nr database blast for comparison.
Furthermore, we analyzed the functional features of these transcripts according to a part and/or an instance of the parent of gene ontology (GO) terms and BLAST search results of transcripts without GO terms.
The integrated results of transcript and metabolic profiling, for the first time, provided insights into an HR+ type of resistance in rice against gall midge.
The results of transcript analysis were compared with Hsp70 protein expression by immunoblot analysis for validation.
Additional file 11: Contains a Microsoft Excel spread sheet with detailed results of transcript and gene co-expression analysis of Class I and Class II SDH in plants.
Recently, we reported on the results of transcript profiling and single feature polymorphism (SFP) detection in alfalfa using the Medicago GeneChip as a cross-species platform [ 25, 31].
The less than 23% of reads were unmapped probably as a result of unidentified transcripts [ 33].
The resulting subset of transcripts was screened against the SWISS-PROT and TrEMBL protein databases using BLASTx with a cutoff of 1.0×10−20 to assign putative functions to unigenes.
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