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The lack of efficacy in IPC298 cells following BRAF knock-down could be the result of mutant RAS engaging other RAF family member like CRAF.
ASPCR also can provide an accurate quantitative result of mutant percentage in a mixed population, as described (11 ).
Despite decreased DNA repair as the result of mutant BRCA1 expression, this construct produced increased survival in breast cancer cells with DNA double-strand breaks.
These results indicate that despite decreased DNA repair as the result of mutant BRCA1, this construct also produced increased survival in breast cancer cells with DNA double-strand breaks.
To confirm that activation of PERK-eIF2α-P signalling is indeed the result of mutant tau expression in rTg4510 tauP301L+ mice, we tested the effect of repressing tau levels on eIF2α-P and ATF4 levels using doxycycline administration.
Without testing the WT Seipin transgenic mice, it is not possible to ascertain that the observed differences were the result of mutant Seipin overexpression, and not a nonspecific effect due to the overexpression of an exogenous protein.
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Besides a list of missense mutations at gene and protein sequence levels, GALT-Prot reports the analysis results of mutant GALT structures.
Results of mutant were normalized to wild type SERCA activity.
Figure 1A showed the results of mutant screening by gradient PCR using D112K forward and reverse primers which contained two designed mutations.
The results of mutants were compared with that of WT animals assayed in parallel.
Based on the results of mutants synapse and cleavage analysis, we have found more functional conserved residuals in the N-terminus of phiC31 integrase.
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