Consistent with this result, Flag-Sox2-ΔHMG, which contains the C-terminal as well as the N-terminal domains of Sox2, successfully co-immunoprecipitated Sall4 even though its amount in the immunoprecipitation eluate was the lowest among all the different Flag-Sox2 proteins used in this study.
For decades, students granted accommodations on the standardized tests had their results flagged with the notation "Scores Obtained Under Special Conditions".
Having bought a drug on the internet and injected it into his penis, with no resulting "flag up the pole" (Carl's language was instructive), he reluctantly agreed to visit "sacred sexual healer", Larissa.
Bournemouth, playing in front of their highest home crowd of the season, introduced Ritchie and Fraser and that nearly paid dividends as Ritchie had a shot deflected for a corner and blasted another effort wide from the resulting flag-kick, while the other two Cherries replacements combined as Fraser headed Wes Thomas's cross wide.
These contradictory results flag the need for further investigation into the mode of action of these RPs in p53 regulation.
For example, task T4 was "Find all patients who had at least 2 creatinine lab results flagged as too high".
The resulting Flag-MTA1, MTA1(1–460) and MTA1(454–715) protein complexes were subjected to in vitro pulldown assay using immobilized H3 peptides.
GST was cleaved from FLAG-SH using Pre-Scission protease (Amersham Biosciences), and the resulting FLAG-SH protein was solubilized in 0.5% w/v N-laurylsarcosine (Sigma-Aldrich).
The infected HeLa cells were sorted by anti-IL-2R monoclonal antibody (mAb) conjugated with magnetic beads, and the resulting Flag-HA-survivin or Flag-HA-T34A survivin stable cell lines propagated as suspension cultures.
As a result, major flag carriers have been forced to cut back.
