With this technique, a foreign gene (i.e., transgene) is introduced into a recipient organism's genome, resulting in a transgenic animal.
This resulted in a transgenic line termed 3D7attB::PfSCD-GFP, in which the Pfscd-gfp sequence was integrated into the chromosomal attB site (Fig. 3A).
Three of the four adaptations of Rokyta and Wichman (2009) also resulted in a transgenic fitness equal to or above that of one of the adapted parent genomes.
Notably, increased expression of CK2α in the mouse mammary gland under control of the MMTV-LTR resulted in a transgenic mouse model of breast cancer [ 49].
The two regions have been physically separated and when one region was bred into an autoimmune resistant strain, it resulted in a transgenic mouse with Sjögren's-like salivary gland disease but without pancreatitis or diabetes [ 15].
Although molecular mechanisms of heat stroke under physiological and pathological conditions have not yet been elucidated, a novel disease-associated gene encoding a calcium-binding protein, calsequestrin-1 (CASQ1), was suggested relevant based on results from a transgenic murine model.
These data are in contrast to results from a transgenic mouse model, where the efflux properties of brain endothelial cells were reported to be impaired in glioma tissue (Bleau et al., 2009).
Our DNA FISH results in a transgenic mouse line harboring two additional transcriptionally active copies of the PWS-IC through MBII-85 at another genetic locus revealed that transcriptional activity alone was insufficient to induce full chromatin decondensation, further implicating the importance of snoRNA cluster genomic neighborhood in chromatin remodeling and decondensation.
Forced overexpression of DDAH-1 in transgenic mice resulted a 2-fold reduction in plasma ADMA levels and decreased blood pressure [ 32], while the loss of DDAH-1 activity by homologous recombination in mice led to accumulation of circulating ADMA and increased blood pressure [ 33].
We used a transient expression system which does not result in transgenic plants.
