Furthermore, in 1984, responding to a backlash against restrictive cuts imposed in the Social Security Disability Amendments of 1980, policymakers expanded the ways in which a person could medically qualify for the DI program.
We accepted the truncated power law as a better model after imposing a restrictive cut-off of four AIC points.
Even after choosing a restrictive cut-off of p<3·10−4 (corresponding to a 0.05 significance level after a Bonferroni correction for multiple testing), we find that 77 (out of the 150) miR-seeds have a significant increased activity in tumors (Table S4).
As significance testing was not possible for this analysis approach, restrictive cut-offs were used instead.
Our general approach with a restrictive cut-off favors genes with either a consistent or a very strong alteration.
Fourthly, we focused on either highly significantly changed genes (P < 0.01) or used restrictive cut-offs (log2 fold-changes >4 or <−4.5).
Results do not change significantly when using a less restrictive cut-off criterion to select the peaks included in the quantitative analysis.
Although the sample size of our study could result in low statistical power, the robust statistical analysis used, including univariate and multivariate analysis, and restrictive cut-off points, have helped to identify some relevant variables related to long-term outcome.
A restrictive cut-off of 95% of identity in at least 50 bp was established to guarantee that only similarities at the level of nearly identical microbes were counted.
A restrictive cut-off of 98% of identity in 90% of the length of the environmental read was established to guarantee that only similarities at the species level were counted.
