He was however bounded by a restriction: to select only a Roman citizen for the position.
These assays were performed across the long arm of homology (Fig. 3B) using SalI and Bgl2 for restriction and the short arm of homology (Fig. 3C) using BamHI for restriction to select clones that had experienced homologous recombination at the Smyd2 locus.
There is no critical restriction to select a target site other than the target length and the presence of a PAM in the CRISPR/Cas9 system.
To this end, we carried out a search in Pubmed/MEDLINE (time restrictions: 2006 October 2014) to select key publications in the English language, using a combination of the following keywords (free text search strategy): 'preterms', 'preterm neonates', 'NICU'drugrutilization'on'drugruseuse'AKIAKI'drug-inducedced kidney injury', and 'drug-induced renal damage biomarkers'.
To do this, we made a heteroplasmic line containing the wild-type genome and the temperature-sensitive double-mutant mt:ND2 del1 + mt CoI T300I, and used expression of restriction enzyme to select against wild-type genome and used the temperature-dependent selection against the double mutant.
Xu et al (2014) developed the CAPS marker using SduI restriction enzyme to select OsSPL14-Ri22 allele, which transcribes OsmiR156-resistant OsSPL14 transcripts caused by the nucleotide substitution at the OsmiR156 target site.
Clones from each construct were digested with restriction enzymes to select those with a sense orientation and were subsequently sequenced on an ABI 3700 (Applied Biosystems Inc., Australia) at the Australian Genome Research Facility (Adelaide, Australia) to verify the identity of genes and the precision of constructs.
This further restriction is meant to select among the different types of the realisations of the same potentialities.[14] As Aristotle stresses these are the incomplete actualities belonging to these potentialities, because what is actualised in a process of realisation is an incomplete potentiality only (Physics 3.2, 201b32 33).
The age restriction was applied to select patients who had finished education.
We used techniques of restriction and matching to select the healthiest segment of the general population.
