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Due to the restrictions of sample collection, plasma samples were collected from HS, while serum samples were obtained from CHB patients.
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Further restrictions of sampling include data collected only in one region of Sao Paulo and the exclusion of PHPs on leave and off work, for instance, due to stress-related health conditions.
Because of the restriction of sample conditions and of testing apparatus, push testing is usually used instead in the laboratory.
In reality, both the characteristics of the sample and regression findings are negligibly affected by the restriction of sample size, as further discussed in Results.
To address the restriction of sample to complete cases, predictors for higher attrition were used as a guide to create subsamples.
Although our results are in part corroborated by published knee OA literature, we emphasize that the restriction of sample size as well as modest reliability for radiographic measures may influence the interpretation; replication of these findings within other groups of subjects is recommended.
In general, the results of association studies must be treated with some caution, as they cannot prove causal relationships, and because of practical restrictions on sample size, they often have poor statistical power (Yen et al. 2013).
This evidence justifies the use of KLIPS 2008 as a representative sample and restriction of samples based on the age information from KLIPS 2008.
Traditional forward scattering CDI has some limitations, including the restriction of samples being finite-size and the difficulty of convergence of the phasing algorithm.
Strengths of the present study include the large sample size capturing a wide range of age span from extremely preterm infants to older adults, and the restriction of samples to individuals in the absence of with disease or infection.
However, this study was also limited by the range restrictions of the sample and the limited factors investigated.
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