Sentence examples for restrictions in front of from inspiring English sources
And nobody has put any restrictions in front of me".
Yacoubian is one of 10 shop owners on the block who signed a petition asking the city to crack down on parking enforcement and add tougher parking restrictions in front of their businesses.
I used to chide Ziauddin about these restrictions, especially in front of Malala.
The restrictions limited rallies in front of City Hall to just 50 people on the steps and 150 in the front plaza.
Detroit doesn't place burdensome regulations on automobile manufacturers; Idaho doesn't put undue restrictions and hurdles in front of potato farmers; and California takes steps to protect its farmers — because these industries are part of the lifeblood and identity of their respective states.
The amplified leader sequences were cloned by restriction and subsequent ligation in front of egfp (enhanced GFP; BamHI/ HindIII) into the multiple cloning site of pPha-NR (GenBank JN180663; EcoRI/ HindIII), which allows induced expression of the constructs under control of an endogenous nitrate reductase promoter.
The plasmid for the expression of TBD fused to synaptobrevin was prepared by introducing the Rattus norvegicus synaptobrevin 2 sequence (codon optimized for E. coli expression and synthesized by Eurogentec, from amino acids 3 84 of the NCBI reference sequence NP_036795.1) into the XhoI restriction site of pGEX-KG in front of the TBD sequence in the above pGEX vector.
Additionally, SmaI restriction site and Alanine codon were incorporated in front of the InteinCBD to allow for cloning of blunt-ended inserts, and to improve efficiency of the intein self-cleavage, respectively (described in Experimental Procedures).
Commissioner Charles H. Ramsey and Mayor Nutter announced the restriction of duty at a news conference late yesterday afternoon in front of Police Headquarters.
In front of news cameras, Finicum helped destroy a government fence to protest against restrictions on cattle grazing.
