Pearson correlation coefficient analysis was performed to determine whether the expression frequency of TCR repertoire correlated with its restriction frequency in allochimeric molecules-induced tolerant recipients.
A variety of factors known to influence epitope recognition, including HLA restriction, frequency, and peptide binding capabilities, HIV genetic diversity, and processing-related factors (TAP binding, proteosomal cleavage), were investigated.
Two-sample t-test analysis of the differences of the detection frequency of Vβ restricted in total TCR repertoire between CsA and allochimeric protein-induced tolerant rats showed 6% restriction frequency in CsA-treated tolerant allograft versus 36% in allochimeric protein -treated allograft (Fig. 3B).
Two-sample t-test was applied to detect the differences of restriction frequencies of TCR repertoires in CsA and allochimeric protein treated rat tolerant models.
As summarized in Figure 3B, the restriction frequencies of individual Vβ genes varied in grafts from allochimeric protein-treated tolerant rats: the Vβ4, 8.3, 10, 12, 16 were slightly restricted in most of the transplants and Vβ7, 11, 13, 14, 15 displayed dominant restricted bands, while Vβ1, 3.3, 8.2, 9, 19 were expressed much less frequently (Fig. 3A and 3B).
In addition, the restrictions of frequency resources in those schemes result in a high blocking probability.
A complete description of the dispersion relation with no restrictions on frequency and wavelength is provided.
No restrictions on frequency or duration of training were made.
The explained variances of certain child characteristics (i.e., feeling of isolation, activity restrictions and frequency of daily hassles affecting personal life) were high.
No restrictions on frequency of changes in exposure status were imposed, and the exposure status changed as often as the drug purchases indicated.
