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The cellular proteome is a highly interconnected protein network that is among other restrictions dependent on resources such as space, metabolites and unbound water to allow protein diffusion.
Variants were preferentially selected if they were exhibiting stable category assignments (Additional file 2: Figure S2) with clearly separated clusters to avoid restrictions dependent on the inbred-level.
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The iron restriction dependent membrane perturbations led to an increase in passive diffusion and drug susceptibility.
This suggests that there is restriction-dependent selection for the transposition products (against the R+ parental clones).
Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations.
In other examples, Gis1 was suggested to act as a transcription factor regulating chronological lifespan; calorie restriction-dependent lifespan extension is mediated by the down-regulation of both the Ras and Tor-Sch9 pathways and the resulting activation of the Rim15-controlled Msn2/4 and Gis1.
Age restrictions apply – dependent on the tour chosen – but the minimum age for the family tour is eight years old.
Red edge excitation shift (REES) measurements reveal that the immediate environment of tryptophans in membrane-bound BLA is restricted, and the restriction is dependent on membrane lipid composition.
Further studies confirmed that Vitamin D induced M.tb growth restriction is dependent on the induction of cathelicidin [19].
Flow measurements underneath Caulerpa and seagrass canopies showed that the degree of flow restriction was dependent on the physical structure of the organisms [20].
In other Chordates, 5948 different species had putative 16S amplicon sequences and a range 0 1% of species contained the Pac I restriction site, dependent on the group (Table 1).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com