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This restriction was confirmed during the pontificate of John Paul II.
HLA-DR restriction was confirmed using anti-DR antibodies that significantly inhibited 15HEL responses (Fig. 2B).
HLA restriction was confirmed with peptide-pulsed, heterologous target PBMC matched at a single HLA allele.
Once the optimal epitopes were determined, HLA class I restriction was confirmed in the same assays with peptide-pulsed, heterologous targets matched at a single HLA locus.
The restriction was confirmed by loss of the cytotoxic effect in the presence of anti-HLA-DR and preservation of the effect when anti-HLA class I was used.
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Adherence to rotation and over-hang restrictions was confirmed by an independent observer (MF) at the completion of each templating session.
Although some alternative HLA-I restrictions were confirmed experimentally, the majority were not identified, but inferred by statistical methods [22].
Homeolog loss (versus loss of a restriction site) was confirmed via sequencing.
The tandem organization of the minisatellites lacking restriction sites was confirmed by sequence analysis or PCR (not shown).
Moreover, the presence of specific polymorphic alleles as detected by restriction analysis was confirmed in each case by sequencing of random samples of patients.
We compared IGS size index based on SB and PCR for the 29 species for which presence of one restriction site was confirmed.
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