To balance the reported weaknesses as well as to increase the strength of the current investigation, a strict inclusion-exclusion protocol was applied, according to which specific restrictions to the study designs of the included trials, the participants' characteristics and the principal outcome measures were imposed.
The validity of this published analysis has also been questioned, as restriction to the more current studies eliminates the reported benefit of echinocandin therapy [ 40].
There was no restriction to the type of studies —that is, we included randomised controlled trials (RCTs), non-randomised controlled clinical trials (CCTs), case series/reports or surveys if they contained unique data.
To determine the available shrub area, the legal, technical, economic and environmental restrictions pertinent to the study areas were identified and their relative thresholds were established.
The restriction to a study group below the age of 50 was a prerequisite for the effective exclusion of phenacetin use, and we believe that this is the first study to completely avoid phenacetin contamination in the assessment of ESRD associated with analgesic uses.
The high proportion smear positive in this study may be partly attributed to restriction of the study to those who were smear or culture positive, and to examining smears in a research environment using fluorescence microscopy, which may not be readily available under routine health services and which has been shown to be more sensitive than ZN microscopy [ 19].
Furthermore, owing to the low incidence of VIN 2/3 and VaIN 2/3 and the restriction to one study site, only 94 patients were included in the analysis.
In addition, we examined the sensitivity of the lag pattern to adjustment for gaseous copollutants, including carbon monoxide, sulfur dioxide, nitrogen dioxide, and ozone, to restriction of the study area to Denver County, and to the use of fewer degrees of freedom (4 df and 6 df/year) for time.
One restriction to our study is the chronological control of the sample.
In addition to the noted debate, a further restriction to our study was relating to the use of WHR, especially in women for cultural reasons, to further explore the risk of cardiovascular disease using other parameters.
