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The over-expression of boBST-2 in BEK cells and the deletion of IE1 in BoHV-4 genome should amplify the BoHV-4ΔIE1 phenotype, in terms of restriction release of BoHV-4ΔIE1 IRVPs into the infected BEK-boBST-2 cells supernatant, if BoHV-4IE1 was a real BST-2 counteracting factor.
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NotI/AvrII restriction releases the ama1 promoter in this vector and generates a 3′ replacement constructs that, only after integration, leads to a detectable Rh2b HA product.
He took office on March 30, and his government embarked on an agenda of political and social reforms, including relaxing press restrictions, releasing political prisoners, and concluding cease-fire accords with rebel ethnic groups.
BRUSH SISTERS FILE PLEA.; Ask Court to Lift Restrictions on Release From Hospital.
Archives|BRUSH SISTERS FILE PLEA.; Ask Court to Lift Restrictions on Release From Hospital.
He wrote to Diane Auer Jones, a deputy undersecretary in the Education Department, asking the agency to ease the cash monitoring restrictions and release $13 million to cover the financial aid obligations.
Cognitive radio wireless sensor networks enable a dynamic channel selection with the restriction of releasing a channel when a primary user is present.
Executives there say they are particularly concerned about the two-hour restrictions for release of the photos after a game because the shelf life of news images is so brief, particularly in different time zones.
Olguin put restrictions his release, which would only be granted if $1.6 million was paid in full for the bail.
A third restriction enzyme ("releasing enzyme 2") is applied, generating pool 2 (Fig. 1A, step 3).
A second digestion is performed with another restriction enzyme ("releasing enzyme 1"), and the DNA fragments are collected for amplification as pool 1 (Fig. 1A, step 2).
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