Exact(32)
PCR and restriction products were resolved on 2% agarose gels (Amresco, Solon, OH).
Genomic DNAs from Klebsiella pneumoniae (ATCC700603 and twelve other isolates), Klebsiella oxytoca (one isolate) and Escherichia coli (ATCC25922), were digested by two restriction endonucleases (BsrSI and Fok I), and restriction products were separated on an agarose gel.
The restriction products were fractionated on 2% agarose gels (Agarose, MP, Sigma) with TBE buffer (0.089 M Tris, 0.089 M boric acid and 0.5 M EDTA, pH 8.0), stained with ethidium bromide, and observed under UV light.
Restriction products were separated on a 1.0% agarose gel in 0.5×Tris-Borate-EDTA buffer (TBE) in the Stratagene Rotating Agarose Gel Electrophoresis variant of Pulsed Field Gel Electrophoresis (PFGE) at 12°C and with a rotation angle of 120°.
Restriction products were separated on 4% agarose gel.
Restriction products were then analyzed by 3% agarose gel electrophoresis.
Similar(28)
The MluI/ SalI restriction product was subcloned into p1017D.
1 μl of the restriction product was loaded onto the chip.
The NotI/ SalI restriction product was subcloned into pEFBos rCD2p110, replacing p110 cDNA.
The restriction product was then ligated to a PstI adapter and amplified by PCR using a primer complementary to the adapter sequence.
A 10 μl aliquot of restriction product was separated by gel electrophoresis on 2.0% agarose (Carl-Roth) gels in 0.5× Tris borate EDTA buffer for 3 h.
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