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Exact(27)
Restriction patterns of the spontaneous Hprt mutants were indistinguishable from the wild type pattern, as these mutants probably contain only small deletions or even point mutations in the Hprt locus.
The labelled 44-kb Sma I restriction fragment derived from S. aureus NCTC 8325 4 was hybridized to theEco RI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus.
Hybridization studies with a range of Bacteroides CTn's encoding tetracycline resistance revealed a great deal of homology between most of the CTn's but there was much variation seen in the restriction patterns of these elements, suggesting great diversity among this group.
The validity of the genome assembly was assessed by comparing the agarose gels' restriction patterns to the computer-predicted restriction patterns of the consensus sequence.
These post-entry restriction patterns of TRIM5α orthologues suggest that lentiviruses have evolved to evade TRIM5α-mediated post-entry restriction when colonizing respective species.
Compared to the AscI restriction patterns of S. Albany strain 7205.00, new bands of higher molecular weight appeared in both the AscI digested DNAs of wild type and ΔthdF mutant S. Typhimurium LT2 transconjugants (Fig. 4A).
Similar(33)
The identification procedure described in this paper was validated by establishing a correlation with an Alu I restriction pattern of the different full length amplified 16S rDNA.
The development of the in silico approach led to the creation of a novel dichotomous key which was able to unequivocally identify the eight potato S-RNase alleles already known and eventually detect putative alleles when the size and/or the restriction pattern of the amplicons were different from those predicted.
Additional file 1: Restriction pattern of different PCR products.
When the restriction pattern of the cloned rDNA was compared with that of total rat DNA, the rDNA regions of both Sprague-Dawley and BD-9 rats were identical to each other and to that of the cloned rDNA.
Lane M represents 1 kb ladder; (D) The restriction pattern of cosmid clones no. 957 (Lane 2), 946 (Lane 3), 1956 (Lane 4), 1881 (Lane 5), 88 (Lane 6), 1 kb ladder (Lane M1), λ HindIII digest ladder (Lane M2).
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