16 As an alternative to complete restriction of interactions, some jurisdictions have attempted to regulate them.
The restriction of TNAP interaction with PrPC to neuronal 1C115-HT and 1C11NE cells prompted us to investigate whether TNAP was present in microdomains of 1C11 precursor cells.
Thus, neither the hypomethylation in LADs nor gene expression changes during culture expansion seem to be triggered by extension or restriction of chromatin interaction sites with the nuclear lamina.
Many of them follow restrictions on interaction with nonrelatives, and the head coverings most of them wear make it impossible to blend in.
Isolation of communities and restrictions on interaction between communities, that could be a protective factor against HIV transmission, seem to be less applicable in this context.
Our findings reveal a novel regulatory event that is essential for accurate meiosis I chromosome segregation and demonstrate that temporal restriction of microtubule kinetochore interactions is instrumental in transforming mitosis into meiosis.
In next section we present a fast heuristic algorithm to predict RNA-RNA interaction without applying any restriction on type of interaction and energy model.
Another (simpler) alternative to avoid the restrictions of the interaction networks would be to, for example, allow for each second interaction to take place between arbitrary pairs of (non-adjacent) users.
Intracellular factors might also restrict spreading, such as restriction of PrP synthesis, interactions of PrP with intracellular molecules, modifications to PrP such as glycosylation or cleavage (Goh et al., 2007; Haigh et al., 2009; Kuczius and Kelsch, 2013; Moleres and Velayos, 2007), reuptake of misfolded PrP from the cell surface, or restricted production of exosomes or nano-tubes.
However, such analyses did not take into account the restriction in the number of interaction events that could occur over the course of a typical infectious period.
