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Through a literature search, we identified which putative target genes are expressed in neuronal tissues and with expression analysis, we verified the restriction of expression of four F-box genes to neuronal tissues.
In adjudicating such circumstances, we should follow the principle that, if any restriction of expression is necessary, a restriction should be as limited and narrow in scope as possible.
We all have horrible images in our minds of what the military do when they take power: arrests, interrogations, martial law, authoritarianism and restriction of expression being the least of the evils.
eIF4A may have a particular role in neuronal translation, as suggested by the restriction of expression of BC1/BC200 RNA to neuronal tissue, where it is thought to interact with and inhibit eIF4A [33].
These derive directly from the action of MET1 on the sporophytic vegetative tissues and indirectly from the restriction of expression of imprinted inhibitors of seed size to their maternal allele by MET1 acting during male gametogenesis.
GNA11 showed moderate expression in normal epithelium, without any clear gradient or obvious restriction of expression.
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Meanwhile, concerns have grown to include wider grievances of the Oromo people, including overall restrictions of expression for the ethnic group.
Except for occasionally a few patches, we could not detect β-catenin expression in the mesenchyme of E14.5 CKO lungs and consistent with the restriction of Cre expression to Dermo1Cre/+ mesenchyme, epithelial β-catenin expression appeared unperturbed.
This point is of concern as several known dynamin1-interacting proteins demonstrate restriction distributions of expression as well [27], [28].
Restriction sites for BamHI and HindIII were included at respectively the 5'and 3'end of the coding sequences to enable cloning in the corresponding restriction sites of expression vector pET15new.
Furthermore, adult mice were used in the EAE experiment and for the regional expression study, arguing against a developmental restriction of EAAT2 expression regulation in our study.
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