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We further asked whether their appearance was dependent on the restriction function or not.
In other words, there appeared strong dependence on restriction function, to which we will come back after examination of structure of these products.
In order to examine dependence on the restriction function, we performed experiment series 2. Figure 3A (experiment series 1) shows that the stop of the viable cell count because of the post-segregational killing in the R+ culture allowed us to detect the transposition clones.
Although sometimes effective, the loss of restriction function is always associated with loss of modification function, which means the phenotype will be reversed when the cells recovered from abnormal conditions; Inactivation of the RM system [38], which is a clean and preferable method, but often limited by the availability of the genetic tools.
This results from the asymmetry of selection for restoring methylation function and restriction function after the corresponding genes are fragmented.
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By Lemma 2.2, C ( J, C n ) is a Banach space, the restriction functions of x ( s ) in J k must be convergent.
So, for every k, we have { ∥ x ( s ) − x ( l ) ∥ J k C } < ε, the inequality (4) shows that the restriction functions of x ( s ) in J k are the Cauchy series of C ( J, C n ).
The results indicate that nicotine might, via altering the expression of cyclin D1, lift DNA damage-induced growth restriction, and function as a rate limiting factor of the G1 restriction in nicotine-mediated cell cycle progression.
Perceived restriction of function: The Funktionsfragebogen Hannover (FfbH-R) consists of 12 items that ask about the patient's capacity to perform daily life activities.
Phosphorothioate DNA modifications incorporated by Dnd proteins (DndABCDE) that work together with the DptFGH- restriction system function as a restriction-modification (R-M) system, which is evident in E. coli B7A [ 26].
It is surprising that Walker-Bone et al. found adhesive capsulitis in 55% of the cases because this is a disease regarded to cause a lot of pain and restriction in function.
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