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Because homologous recombination is very rare or absent in influenza viruses [31], [32], this restriction does not alter the results of our analysis.
Lifelong caloric restriction does not alter age-associated knee OA in 24-month old mice, but it does delay the development of hip OA in dogs [ 64, 65].
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Chronic moderate sleep restriction did not alter anxiety-related behavior, but exercise training significantly attenuated anxiety-related behavior.
Calorie restriction did not alter muscle AKT2 protein abundance in either phenotype, but total AKT2 protein was elevated in GHRKO AL mice compared to normal AL controls (P<0.05, Figure 4F).
Sleep restriction did not alter plasma NEFA levels during the clamp (143 ± 29 vs. 133 ± 29 μmol/l, NS).
A lack of a significant effect was also observed in another study in which a 4-week protein restriction did not alter bone strength [ 3].
The p.M1? variant does not alter restriction site, therefore, a reverse primer E1_M1T-R was designed and used with forward primer E1-F to yield PCR product containing the mutated site and to create an artificial EagI restriction site (C′GGCCG) in the p.M1? allele.
The mutation in purC does not alter a restriction enzyme site so could not be assessed via PCR-RFLP.
We have shown that blocking pore conductance does not alter AmphoB's effects on restriction.
While the final set of restrictions severely cut the sample size (16,798 observations left), this does not alter the mean age and educational attainment of those 16 18 year-olds living with both parents.
Time does not alter that message.
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