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Our results suggest that ArdA can overcome the restriction barrier following conjugation and so helps increase the spread of antibiotic resistance genes by horizontal gene transfer.
The antirestriction proteins, ArdK and ArdR, may play a role in overcoming the host restriction barrier by self-transmissible broad-host range plasmids.
All deletions were generated in F. novicida mutant strain MFN245, a quadruple mutant that substantially reduces the restriction barrier and thereby increases the efficiency of transformation [44].
To overcome the uncharacterized restriction barrier, we attempted to mutate the unknown restriction recognition sequence in the 334 bp inhibitory region of pSY6catP.
One possibility is that COG4636+ members serve as a part of the restriction barrier, similarly to the unrelated NucA family of extracellular nucleases found in Cyanobacteria, e.g. Anabaena sp. PCC 7120 [ 52] and Microcystis sp. [ 53].
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Already, height restriction barriers have been built over roads throughout Kabul to block trucks.
Worries over children accessing pornography have led to calls in the UK for age restriction barriers on all adult sites - but critics say such a policy is difficult to enforce as most sites are based overseas.
Indeed, Ramay y Vendelin [29] developed a three dimensional finite-element mathematical model in which the restriction barriers were composed by the sarcoplasmic reticulum, cytoskeleton proteins close to this reticulum and cytoskeleton proteins crowding that well adjusted the existent experimental data.
"Mexico could put up certain restrictions, barriers, slow-walk it," Olson said.
"CalOptima has new restrictions, presenting barrier to new enrollees, with single adults often ineligible.
Both countries, he complains, have erected "all kinds of restrictions and barriers" to shut out American products, including beef and automobiles.
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