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Combined bisulfite restriction analysis.
amplified ribosomal DNA restriction analysis.
Correct methylation was checked by restriction analysis with Fnu 4HI.
Open image in new window Fig. 2 Bisulfite sequencing, Methylation Specific PCR and Restriction Analysis.
To confirm the results, the restriction analysis for the uncut C allele was repeated.
Successful transformation was checked by a colony PCR as well as a restriction analysis.
Orientation and codon in-frame for all constructs were confirmed by restriction analysis and sequencing.
The microorganism community structure was analyzed by amplified ribosomal DNA restriction analysis.
All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis.
16S rRNA and DNA PCR products were cloned and screened by restriction analysis.
Constructs were confirmed by restriction analysis and DNA sequencing.
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