Sentence examples for restriction accessibility from inspiring English sources

Restriction accessibility assays were performed with salt dialysis-reconstituted chromatin with Hae III restriction enzyme and the indicated concentrations of either yChd1 or hBrg1.

The ideal sensor for robot application should measure the welding point (avoidance of tracking misalignment), should detect in advance (finding the start point of the seam, recognizing corners, avoiding collisions), and should be as small as possible (no restriction in accessibility).

To test this further, restriction enzyme accessibility assays (REAAs) were done to determine whether iAs affects chromatin remodeling, an event associated with initiation.

Restriction site accessibility in wt and cyc8 nuclei for the enzymes and concentrations indicated.

Restriction enzyme accessibility assays were performed as described previously (Alexiadis and Kadonaga, 2002; Rattner et al., 2009).

To address this question, we have used an entirely purified in vitro system and a restriction enzyme accessibility assay (REA).

Very remote areas are defined by geographic distance which imposes a severe restriction on accessibility to a range of goods and services [ 37].

Furthermore, restriction enzyme accessibility assay results were not consistent with digestion of naked DNA (Supplemental Fig. S2), suggesting the remodelled region was not completely devoid of nucleosomes.

The pUC19 plasmid was linearized with HindIII, to eliminate the effect of different plasmid conformations on restriction enzyme accessibility and facilitate site mapping during the DNA protection assay.

Chromatin restriction enzyme accessibility was assayed as previously described [48], hybridising with probes − 1283 to − 1054 bp (SpeI) and − 1670 to − 1878 bp (SpeI and RsaI).

However, in cyc8 mutants, the promoter nucleosome positioning is lost resulting in a dramatic expansion of the hypersensitive site and increased restriction enzyme accessibility to the underlying DNA.