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To get a comprehensive view about the gene evolution, we performed multiple separate blast searches by restricting the database to the sequences from fungi, animals, and other eukaryotic groups or by excluding the sequences from land plants and/or fungi, and the sequences from representative species were added to the previous dataset.
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We note whether a tail check was performed, but it is not currently possible to restrict the database output for paleointensity experiments that incorporated this check.
We note in the database whether either of these approaches have been applied and it is possible to restrict the database output to only paleointensity results corrected for cooling rate dependence ('General constraints' section).
The tBLASTx searches were performed in a local Linux workstation against the complete nematode EST database with the -l option enabled to restrict the database search to the list of GI's of the targeted group [ 7].
After choosing a data set, users can construct queries using the basic BioMart concepts of attributes, which indicate what information should be returned, and filters, which restrict the database entries that are retrieved.
At present we have restricted the database of potential target sequences to those from 3' UTRs, but this could be expanded to include entire mRNAs given evidence that miRNAs bind to other regions of animal mRNAs.
The version of dbWFA presented here is operational, but the aim is not to restrict the database to storing O. sativa and A. thaliana annotations but to expand it to include data from other plant species genomes as their functional annotation becomes more consistent.
Such problems may be circumvented by restricting the experimental database to point FRFs; in this case, the procedure is quite robust and excellent results are obtained, although it is necessary to plan experimental testing differently from the usual modal testing, with possible impact on related costs.
As the overlap with TFBSs is only putative, we made the prediction more likely to be of biological importance by restricting the Transfac Matrix Database (v7.0) TFBSs to those conserved in the human/mouse/rat alignment (http://genome.ucsc.edu/cgi-bin/hgTrackUi g=tfbsConsSites).ucsc.edu/cgi-bin/hgTrackUi g=tfbsConsSites
The trade commission voted 3 to 2 last month in favor of a settlement restricting the sale of the database.
This procedure allowed us to restrict the initial database to the portion(s) of TEs that were not covered by any other elements of the exemplar database under our mapping criteria.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com