Acetylation is known to restrict the binding of UX1 to (Me GlcA residues [ 51].
ISW2 function was shown in one study to restrict the binding of the SWI/SNF chromatin remodeler at a target gene in yeast (Tomar et al., 2009).
This suggests that the occupancy of a nucleosome at a potential binding site in GM12878 restricts the binding of Kaiso to that region, contributing to the cell-type-specificity of Kaiso binding.
The binding of proteins to the surface of the array can also be influenced by the amount of ACN in the binding buffer where higher ACN restricts the binding to proteins which are more hydrophobic than the buffer [ 65].
To restrict the binding site of LCAs to N- and C-terminal domains, we used a truncated version of ATGL lacking the C-terminal part of the enzyme (Q289ter).
Molecular studies show that when both ER α and ER β are present together in tumor cells, each ER restricts the binding site occupancy of the other, with ER α generally being dominant to ER β.
Furthermore, results obtained with mouse and cnidarian Hox proteins indicate that the inhibitory activity of SLiMs could be important for restricting the inherent binding potential of intrinsically disordered regions.
This splicing event establishes a selectivity filter to restrict the ligand binding specificity of FGFRb and FGFRc isoforms to mesenchymally and epithelially derived fibroblast growth factors (FGFs), respectively [ 5].
Therefore, in the absence of active transporters, there was a lower bound β-catenin steady state concentration C 0 C in the cytosol-membrane compartment than in the nucleus C 0 N i. e. C 0 C < C 0 N. Note, to achieve B 0 C = B 0 N while C 0 C = C 0 N did not restrict the relative binding affinity or total ligand concentration in the cytosol and nucleus.
Given that NAD+ does not contain the 2'phosphate group, we postulate that the insertion of the two positively charged residues may restrict the adenosine-binding pocket of human SSADH to bind NAD+ rather than NADP+.
Although local conformational changes may well change the precise details and relieve steric hindrance, His is very strategically positioned at one side of the FMN cofactor to restrict the substrate-binding region, a feature that does not exist in bacterial FMN azoreductases.
