Sentence examples for restricted with the from inspiring English sources
Genomic DNA was isolated and restricted with the respective restriction enzymes indicated in each panel.
The plasmid pcdTNFR, in which dTNFR is located downstream of a cytomegalovirus promoter, was constructed by removing the dTNFR gene from pGTTRD by restriction with HindIII and XbaI and inserting it into pcDNA3 (Invitrogen, Leek, The Netherlands) restricted with the same enzymes.
Since plasmid PL463 had been amplificated by PCR, the region between the XbaI-SapI restriction sites was sequenced and then transferred into PL458 restricted with the same enzymes to obtain the vector for the constitutive expression of chimeric LacZ1 8Met-MTGase denominated PLacZ1 8Met-MTGasenel e).
The self-contained regulated plasmid pGTRTT encoding dTNFR was then contructed by removing the P tet dTNFR cassette from pGTTRD by restriction with Nhe1 and PflMI and ligating it into pGTRTL restricted with the same enzymes.
pGTRTE is an autoregulatory plasmid from which enhanced green fluorescent protein (EGFP) expression is regulated, and it was constructed by removing the P tet EGFP cassette from pGTE by restriction with XhoI and PflMI and inserting it into pGTRTL restricted with the same enzymes.
The plasmid pcLuc+, which encodes luciferase from a cytomegalovirus promoter, was constructed by removing the improved luciferase gene (Luc+) from pGL3 Basic (Promega Corp., Madison, WI, USA) with the restriction enzymes XhoI and XbaI and inserting the gene into pcDNA3 restricted with the same enzymes.
It is shown that during charge, water transfer is significantly restricted with increasing the concentration of HBr when the Nafion 115 cation exchange membrane is employed.
The local adaptors cooperatively behave as the centralized one restricted with respect to the specified desired interactions.
The selection is, however, restricted with respect to the capability of forming multi-hop topologies.
The head motion was restricted with foam padding around the head and the noise of the scanner was attenuated with earplugs.
Women were not restricted with regard to the amount they could write, or the length of time they could speak if participating in a phone interview.
